⚖️ Quantifying Western Blot Bands with ImageJ: Step-by-Step Tutorial for Accurate Analysis
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ImageJ is a public domain program developed by Wayne Rasband while at the National Institutes of Health (NIH). In this video I show the NIH-recommend process for quantifying bands derived from SDS-PAGE.
Below are the instructions for using the area under the peak method in your western blot quantitation. This method is useful when you have multiple western blot bands that are distinct from each other. That is, different proteins that cannot be quantified together as an Integrated Density.
The method is also preferred when the bands you need to quantify are very faint.
1. select the rectangular tool
2. draw around the lane you wish to quantify
3. Go to ANALYZE -- GELS -- SELECT FIRST LANE
4. The rectangle will be numbered as '1'. Use the forward arrow of your keyboard to move to the next lane.
5. go back to ANALYZE -- GELS -- SELECT NEXT LANE
Move the rectangle to all additional lanes and SELECT NEXT LANE after each move to register the lane
6. go to ANALYZE -- GELS --PLOT LANES
This shows you individual peaks associated with each protein band in the lane.
7. use the ‘line’ tool to mark the base of each peak
8. use the ‘wand’ tool to select each of the peak. As each peak is selected, a value is given for that peak.
Copy the values and analyse in excel or another spreadsheet program as shown in the previous video.
//PREVIOUS VIDEO USING INTEGRATED INTENSITY IN IMAGEJ: 📊 WESTERN BLOT ANALYSIS using ImageJ...
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/MUSIC
Shine by Joakim Karud
Use my music in your youtube videos: http://joakimkarud.com/use-my-music/
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//About the Video
This video is about western blot analysis with ImageJ, quantifying bands on SDS-PAGE using ImageJ, ImageJ tutorial, Using ImageJ to quantify proteins bands, western blot densitometry in ImageJ, quantifying intensity in imagej, imagej quantify gel bands, western blotting, western blot data, blot data, western data, or Immunoblotting, Western blot quantification, ImageJ software tutorial, Band intensity analysis, Protein quantification in Western blot, Gel electrophoresis analysis, Image analysis software, Molecular biology techniques, Western blot image processing, Protein expression quantification, Digital image analysis, ImageJ gel analysis
Densitometry in Western blot, Band density measurement, Western blot data analysis and
Bioinformatics tools for Western blot.
ImageJ is a public domain program developed by Wayne Rasband while at the National Institutes of Health (NIH). In this video I show the NIH-recommend process for quantifying bands derived from SDS-PAGE.
Below are the instructions for using the area under the peak method in your western blot quantitation. This method is useful when you have multiple western blot bands that are distinct from each other. That is, different proteins that cannot be quantified together as an Integrated Density.
The method is also preferred when the bands you need to quantify are very faint.
1. select the rectangular tool
2. draw around the lane you wish to quantify
3. Go to ANALYZE -- GELS -- SELECT FIRST LANE
4. The rectangle will be numbered as '1'. Use the forward arrow of your keyboard to move to the next lane.
5. go back to ANALYZE -- GELS -- SELECT NEXT LANE
Move the rectangle to all additional lanes and SELECT NEXT LANE after each move to register the lane
6. go to ANALYZE -- GELS --PLOT LANES
This shows you individual peaks associated with each protein band in the lane.
7. use the ‘line’ tool to mark the base of each peak
8. use the ‘wand’ tool to select each of the peak. As each peak is selected, a value is given for that peak.
Copy the values and analyse in excel or another spreadsheet program as shown in the previous video.
//PREVIOUS VIDEO USING INTEGRATED INTENSITY IN IMAGEJ: 📊 WESTERN BLOT ANALYSIS using ImageJ...
.
.
.
/MUSIC
Shine by Joakim Karud
Use my music in your youtube videos: http://joakimkarud.com/use-my-music/
.
.
.
.
.
.
.
.
//About the Video
This video is about western blot analysis with ImageJ, quantifying bands on SDS-PAGE using ImageJ, ImageJ tutorial, Using ImageJ to quantify proteins bands, western blot densitometry in ImageJ, quantifying intensity in imagej, imagej quantify gel bands, western blotting, western blot data, blot data, western data, or Immunoblotting, Western blot quantification, ImageJ software tutorial, Band intensity analysis, Protein quantification in Western blot, Gel electrophoresis analysis, Image analysis software, Molecular biology techniques, Western blot image processing, Protein expression quantification, Digital image analysis, ImageJ gel analysis
Densitometry in Western blot, Band density measurement, Western blot data analysis and
Bioinformatics tools for Western blot.
4 سال پیش
در تاریخ 1399/10/24 منتشر شده
است.
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