Cell cycle analysis

A common question asked in many research papers - after treating cells with some drug or other treatment, what happens to their cell cycle progression? If you take any population of cells (let's say you have 10,000 cells in a experiment), some percentage of them are in G1 phase, some percentage of them are in S phase, some percentage of them are in G2 phase, some percentage of them are in M phase. When treating cancer cells, the goal would be to stop their progression through the cell cycle (for example, there would be less cells going into S, G2, and M phases, and more cells in G1 phase). There are many techniques used by scientists to quantitate stages of the cell cycle. This video covers one type of cell cycle assay using PI. Learning objectives: Know what PI binds to in cell, and when that binding can occur. Explain the different areas (peaks) of a PI cell cycle assay, and how the peaks correlate with cells in different stages of the cell cycle.

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