Plant Biotech/Process of gene transformation with Ti plasmid of Agrobacterium by Dr Dheerendra kumar
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#Discuss the
#Discuss the process of transformation with Ti-plasmid of Agrobacterium tumifacian
#plant biotech
@aktu#aktu
#Crown Gall Disease and Ti Plasmid
#crown gall tumors
#Ti Plasmid
#There are two species of Agrobacterium:
i. A. tumefaciens that induces crown gall disease.
ii. A. rhizogenes that induces hairy root disease
Crown Gall Disease and Ti Plasmid:
Smith and Townsend postulated that a bacterium was the causative agent of crown gall tumors.
A. tumefaciens infects wounded or damaged plant tissues, in induces the formation of a plant tumor called crown gall.
The entry of the bacterium into the plant tissues is facilitated by the release of certain phenolic compounds (acetosyringone, hydroxyacetosyringone) by the wounded sites.
#Organization of Ti plasmid:
The Ti plasmids (size 200 kb each) exist as independent replicating circular DNA molecules within the Agrobacterium cells.
#The Ti plasmid has four important regions-
1. Virulence region
2. T-DNA region
3. Opine catabolism region
4. Origin of Replication
#The Agrobacterium-mediated plant transformations-
#Question- Discuss the method for production of marker free transgenic plant.
Question- How marker free transgenic plant are produces? Explain
5 ماه پیش
در تاریخ 1403/01/20 منتشر شده
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