What is Insertional inactivation? How it helps in the selection of recombinant colonies?

This video explains the process Insertional inactivation in recombinat DNA technology citing pBR322 cloning vector as example.

Insertional inactivation is the inactivation of  a gene upon insertion of another gene inside in its place or within its coding sequence.

This video also explains the selection of recombinant colonies in selective antibiotic medium by insertional inactivation.

• #education
• #biotechnology
• #pbr322
• #insertional_inactivation_in_pbr322
• #recombinant_dna_invention
• #antibiotic_selection
• #recombinant_dna_technology
• #antibiotic_resistance_markers