Loop-Mediated Isothermal Amplification (LAMP): Assay Development Challenges and Solutions

See our LAMP solutions: http://www.lucigen.com/PCR-and-Amplification/ Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method that is rapid, sensitive, accurate, and cost-effective. Due to the isothermal amplification characteristics, LAMP is amenable to assaying diagnostic samples at the point-of-care or environmental samples at the point-of-collection. Over the years scientists have used LAMP technology to develop assays for a variety of infectious agents including bacteria, viruses, fungi and parasitic organisms. They also developed assays for the diagnosis of cancer, identification of invasive species, detection of food adulteration and identification of drug resistance, among others. However, assay development for new targets remains challenging in part due to the complex design requirement of six LAMP primers and the need to optimize buffer conditions. Suboptimal assay design results in slow amplification kinetics, low sensitivity, and poor specificity. This webinar will outline the basic design of LAMP assays and how it is different from PCR. We will discuss key parameters that affect assay performance and approaches to assay optimization. We will also detail common methods to monitor amplification and how to troubleshoot assay failures. Lastly, we will introduce a novel thermostable enzyme suitable for robust and sensitive DNA as well as RNA LAMP. Key Learning Objectives Learn basic design of LAMP assays and the key differences from PCR Learn key parameters that affect assay performance and approaches to assay optimization Explore the common causes of assay failure and how to troubleshoot Discover ways to apply LAMP in point of care/point of collection using a novel thermo-tolerant LAMP master mix that is room temperature stable when freeze dried

• #science_technology
• #loop_mediated_isothermal_amplification
• #isothermal_amplification
• #lamp_assays
• #dna_lamp
• #rna_lamp