Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 + PhiX174)
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Rolling circle mode of DNA replication is a commonly used method to replicate viral DNA molecules, which are circular and often single stranded. This also applies to episomal DNA in bacteria like plasmids, since a lot of them tend to be viral derivatives. Rolling circle replication is also relevant to the method of RCA - rolling circle amplification. In this video, I discuss the contrast between theta and rolling circle mode of DNA replication. The majority of the video focuses on detailing the mechanism by which rolling circle occurs and we discuss two specific examples - M13 and PhiX174 bacteriophages - to conceptually understand the mechanism, and how it can vary between different DNA molecules.
Jump to your favorite section:
00:00 Theta DNA replication
01:16 M13 Rolling Circle Replication
07:45 X174 Rolling Circle Replication
Patreon Page: Patreon: the_Crux
Complete DNA Replication playlist: All about DNA Replication!
Must watch playlists
All videos on DNA Replication: All about DNA Replication!
All videos on Transcription: All about Transcription!
All videos on Translation: All about Translation!
Do leave a LIKE if you find this content useful! COMMENT your questions, suggestions, and thoughts :) And I hope you have SUBSCRIBED because there is a lot more coming! :D
I appreciate your support.
Rolling circle mode of DNA replication is a commonly used method to replicate viral DNA molecules, which are circular and often single stranded. This also applies to episomal DNA in bacteria like plasmids, since a lot of them tend to be viral derivatives. Rolling circle replication is also relevant to the method of RCA - rolling circle amplification. In this video, I discuss the contrast between theta and rolling circle mode of DNA replication. The majority of the video focuses on detailing the mechanism by which rolling circle occurs and we discuss two specific examples - M13 and PhiX174 bacteriophages - to conceptually understand the mechanism, and how it can vary between different DNA molecules.
Jump to your favorite section:
00:00 Theta DNA replication
01:16 M13 Rolling Circle Replication
07:45 X174 Rolling Circle Replication
Patreon Page: Patreon: the_Crux
Complete DNA Replication playlist: All about DNA Replication!
Must watch playlists
All videos on DNA Replication: All about DNA Replication!
All videos on Transcription: All about Transcription!
All videos on Translation: All about Translation!
Do leave a LIKE if you find this content useful! COMMENT your questions, suggestions, and thoughts :) And I hope you have SUBSCRIBED because there is a lot more coming! :D
I appreciate your support.
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