How to Leverage Illumina Sequencing in Genome-Wide CRISPR Screens
2.8 هزار بار بازدید -
3 سال پیش
-
Learn about how Clustered Regularly
Learn about how Clustered Regularly Interspaced Short Palindromic repeats (CRISPR) screens can be an important tool in your research, as well as how to perform a successful experiment using NGS as a downstream readout.
Presenter: Lauren Fait– Sr. Sequencing Specialist, Illumina
00:00 Introduction
00:32 CRISPR Screening Overview
2:28 Natural CRISPR Systems Have 2 Major Components
5:11 CRISPR Screen Overall Workflow
6:49 sgRNA Library Assembly and Packaging
9:04 NGS Quality Control for Pooled Libraries
10:05 Pooled CRISPR Screen
15:12 Genome-Scale Knockout Libraries
18:00 Hit Identification using Illumina Sequencing
20:58 Single-Cell Sequencing for CRISPR Screens
Subscribe to the Illumina video channel http://www.youtube.com/subscription_c...
A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond.
*Data calculations on file. Illumina, Inc., 2015.
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Presenter: Lauren Fait– Sr. Sequencing Specialist, Illumina
00:00 Introduction
00:32 CRISPR Screening Overview
2:28 Natural CRISPR Systems Have 2 Major Components
5:11 CRISPR Screen Overall Workflow
6:49 sgRNA Library Assembly and Packaging
9:04 NGS Quality Control for Pooled Libraries
10:05 Pooled CRISPR Screen
15:12 Genome-Scale Knockout Libraries
18:00 Hit Identification using Illumina Sequencing
20:58 Single-Cell Sequencing for CRISPR Screens
Subscribe to the Illumina video channel http://www.youtube.com/subscription_c...
A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond.
*Data calculations on file. Illumina, Inc., 2015.
View customer spotlight videos
Illumina Customer Testimonials
View Illumina webinars
Webinars
View Illumina product videos
Playlist
View Illumina support videos
Illumina Support
3 سال پیش
در تاریخ 1400/09/22 منتشر شده
است.
2,840
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