PCR technique
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The PCR (Polymerase Chain Reaction)
The PCR (Polymerase Chain Reaction) technique is a widely used molecular biology method for amplifying a specific segment of DNA. Here's a brief overview of how it works:
Denaturation: The DNA sample is heated to separate the double-stranded DNA into single strands.
Annealing: The sample is cooled, allowing short DNA primers to bind to complementary sequences on each strand of the target DNA.
Extension: DNA polymerase synthesizes new DNA strands by extending from the primers along the target DNA sequence.
Repeat: The process is repeated multiple times (usually 20-40 cycles), resulting in exponential amplification of the target DNA segment.PCR is used in various applications including DNA sequencing, genetic testing, cloning, and forensics. It revolutionized molecular biology by enabling the amplification of DNA from very small samples, allowing for easier analysis and manipulation of genetic material.
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Denaturation: The DNA sample is heated to separate the double-stranded DNA into single strands.
Annealing: The sample is cooled, allowing short DNA primers to bind to complementary sequences on each strand of the target DNA.
Extension: DNA polymerase synthesizes new DNA strands by extending from the primers along the target DNA sequence.
Repeat: The process is repeated multiple times (usually 20-40 cycles), resulting in exponential amplification of the target DNA segment.PCR is used in various applications including DNA sequencing, genetic testing, cloning, and forensics. It revolutionized molecular biology by enabling the amplification of DNA from very small samples, allowing for easier analysis and manipulation of genetic material.
#space #motivational #shortvideos #think #cartoon
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