MHC-tetramer Staining and Quantitative Analysis of CD8 T Cells | Protocol Preview
691 بار بازدید -
2 سال پیش
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In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues - a 2 minute Preview of the Experimental Protocol
Shengbin Li, Gwantwa Mwakalundwa, Pamela J. Skinner
University of Minnesota, Department of Veterinary and Biomedical Sciences;
Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues.
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In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues - a 2 minute Preview of the Experimental Protocol
Shengbin Li, Gwantwa Mwakalundwa, Pamela J. Skinner
University of Minnesota, Department of Veterinary and Biomedical Sciences;
Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: jovejournalofvisualizedexperiments
2 سال پیش
در تاریخ 1401/05/12 منتشر شده
است.
691
بـار بازدید شده