Delivery-Cas9/sgRNA Ribonucleoprotein Complex: Immortalized & Primary Cells l Protocol Preview
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2 سال پیش
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Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ("Nanoblades") - a 2 minute Preview of the Experimental Protocol
Philippe E. Mangeot, Laura Guiguettaz, Thibault J. M. Sohier, Emiliano P. Ricci
LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France,; CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France,;
We have developed a simple and inexpensive protocol to load Cas9/single-guide RNA (sgRNA) ribonucleoprotein complexes within virus-like particles. These particles, called "Nanoblades", allow efficient delivery of the Cas9/sgRNA complex in immortalized and primary cells as well as in vivo.
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2 سال پیش
در تاریخ 1401/05/21 منتشر شده
است.
238
بـار بازدید شده