Genome Editing with engineered nucleases
28 هزار بار بازدید -
9 سال پیش
-
From mega-nucleases, to zinc fingers,
From mega-nucleases, to zinc fingers, to TALENs, and now CRISPR-Cas, the use of engineered nucleases for genome editing has been gathering pace. This video outlines these four basic genome editing technologies and gives examples of how edited genomes may benefit us all in the future.
For more information, visit:
- www.thermofisher.com
- www.thermofisher.com/genomeediting
- www.thermofisher.com/genomeeditposters
VIDEO TRANSCRIPT
Genome editing with engineered nucleases
Genome editing inserts, replaces, or removes DNA using artificially engineered nucleases, or “molecular scissors.”
There are four families of designer engineered nucleases:
- Engineered mega-nuclease re-engineered homing endonucleases;
- Zinc finger nucleases;
- Transcription activator-like effector nucleases; and
- Clustered regularly interspaced short palindromic repeats, or the CRISPR-Cas system.
Genome editing with engineered nucleases begins with DNA-specific double-stranded breaks at the target sequence, catalyzed by the engineered nucleases. These double-stranded breaks can be repaired one of two ways.
The first is non-homologous end joining, which occurs when no homologous DNA is present.
Non-homologous end joining leads to insertions, deletions, inversions, and gene disruption, as DNA is ligated randomly.
When homologous DNA is added, the double-stranded breaks are repaired with homology-directed repair. This targeted repair allows for precise gene additions or corrections.
Nuclease-mediated genome editing can be applied in a diverse set of species, including:
- Human cell lines and stem cells;
- Drosophila;
- C. elegans;
- Mice and rats
- Plants;
- Monkeys;
- Zebrafish;
- And many more.
Genome editing has been a growing field since 1969, with over 21,000 publications in the genome editing space.
Genome editing has the potential to profoundly change the field of genetics. From stem cell engineering and gene therapy to disease-resistant transgenic plants, genome editing is bringing exciting changes to the scientific community.
To learn more about genome editing and request a free genome engineering poster for your lab, visit thermofisher.com/genomeeditposters.
For more information, visit:
- www.thermofisher.com
- www.thermofisher.com/genomeediting
- www.thermofisher.com/genomeeditposters
VIDEO TRANSCRIPT
Genome editing with engineered nucleases
Genome editing inserts, replaces, or removes DNA using artificially engineered nucleases, or “molecular scissors.”
There are four families of designer engineered nucleases:
- Engineered mega-nuclease re-engineered homing endonucleases;
- Zinc finger nucleases;
- Transcription activator-like effector nucleases; and
- Clustered regularly interspaced short palindromic repeats, or the CRISPR-Cas system.
Genome editing with engineered nucleases begins with DNA-specific double-stranded breaks at the target sequence, catalyzed by the engineered nucleases. These double-stranded breaks can be repaired one of two ways.
The first is non-homologous end joining, which occurs when no homologous DNA is present.
Non-homologous end joining leads to insertions, deletions, inversions, and gene disruption, as DNA is ligated randomly.
When homologous DNA is added, the double-stranded breaks are repaired with homology-directed repair. This targeted repair allows for precise gene additions or corrections.
Nuclease-mediated genome editing can be applied in a diverse set of species, including:
- Human cell lines and stem cells;
- Drosophila;
- C. elegans;
- Mice and rats
- Plants;
- Monkeys;
- Zebrafish;
- And many more.
Genome editing has been a growing field since 1969, with over 21,000 publications in the genome editing space.
Genome editing has the potential to profoundly change the field of genetics. From stem cell engineering and gene therapy to disease-resistant transgenic plants, genome editing is bringing exciting changes to the scientific community.
To learn more about genome editing and request a free genome engineering poster for your lab, visit thermofisher.com/genomeeditposters.
9 سال پیش
در تاریخ 1394/06/02 منتشر شده
است.
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