AIRR-C Seminar Series, January 25th, 2024 - Stefan Schattgen, St. Jude Children’s Research Hospital
1.3 هزار بار بازدید -
5 ماه پیش
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Spatiotemporal development of the human
Spatiotemporal development of the human T follicular helper cell response to Influenza vaccination
Early Career Speaker: Stefan Schattgen, St. Jude Children’s Research Hospital, USA
Talk abstract
We profiled blood and draining lymph node (LN) samples from human volunteers after influenza vaccination over two years to define evolution in the T follicular helper cell (TFH) response. We show LN TFH cells expanded in a clonal-manner during the first two weeks after vaccination and persisted within the LN for up to six months. LN and circulating TFH (cTFH) clonotypes overlapped but had distinct kinetics. LN TFH cell phenotypes were heterogeneous and mutable, first differentiating into pre-TFH during the month after vaccination before maturing into GC and IL-10+ TFH cells. TFH expansion, upregulation of glucose metabolism, and redifferentiation into GC TFH cells occurred with faster kinetics after re-vaccination in the second year. We identified several influenza-specific TFH clonal lineages, including multiple responses targeting internal influenza proteins, and show each TFH state is attainable within a lineage. This study demonstrates that human TFH cells form a durable and dynamic multi-tissue network.
Speaker bio
Stefan Schattgen is a research scientist and group leader in the lab of Paul Thomas at St Jude Children’s Research Hospital, USA. In 2015 he received his doctorate in immunology and virology at the University of Massachusetts Medical School where he worked on innate immune sensing of viral infections. He turned his focus towards T cell repertoire biology during his postdoctoral training with Paul Thomas from 2015-2020. His current research interests blend computational and wet lab methods for understanding underlying relationships between a T cell’s specificity and their phenotype and functions during homeostasis, infection, vaccination, and cancer.
AIRR-C Seminar Series website: https://www.antibodysociety.org/the-a...
Early Career Speaker: Stefan Schattgen, St. Jude Children’s Research Hospital, USA
Talk abstract
We profiled blood and draining lymph node (LN) samples from human volunteers after influenza vaccination over two years to define evolution in the T follicular helper cell (TFH) response. We show LN TFH cells expanded in a clonal-manner during the first two weeks after vaccination and persisted within the LN for up to six months. LN and circulating TFH (cTFH) clonotypes overlapped but had distinct kinetics. LN TFH cell phenotypes were heterogeneous and mutable, first differentiating into pre-TFH during the month after vaccination before maturing into GC and IL-10+ TFH cells. TFH expansion, upregulation of glucose metabolism, and redifferentiation into GC TFH cells occurred with faster kinetics after re-vaccination in the second year. We identified several influenza-specific TFH clonal lineages, including multiple responses targeting internal influenza proteins, and show each TFH state is attainable within a lineage. This study demonstrates that human TFH cells form a durable and dynamic multi-tissue network.
Speaker bio
Stefan Schattgen is a research scientist and group leader in the lab of Paul Thomas at St Jude Children’s Research Hospital, USA. In 2015 he received his doctorate in immunology and virology at the University of Massachusetts Medical School where he worked on innate immune sensing of viral infections. He turned his focus towards T cell repertoire biology during his postdoctoral training with Paul Thomas from 2015-2020. His current research interests blend computational and wet lab methods for understanding underlying relationships between a T cell’s specificity and their phenotype and functions during homeostasis, infection, vaccination, and cancer.
AIRR-C Seminar Series website: https://www.antibodysociety.org/the-a...
5 ماه پیش
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