The MISSION esiRNA is designed for RNAi screening in mammalian cells
26 بار بازدید -
3 هفته پیش
-
The MISSION esiRNA, also known
The MISSION esiRNA, also known as endoribonuclease-prepared small interfering RNA, is a powerful tool specifically designed for RNA interference (RNAi) screening in mammalian cells. RNAi is a natural cellular process that regulates gene expression by silencing specific genes through the degradation of their messenger RNA (mRNA) molecules.
The MISSION esiRNA is synthesized by in vitro transcription of long double-stranded RNA (dsRNA) templates, which are then processed by the endoribonuclease Dicer into small interfering RNAs (siRNAs). These siRNAs are approximately 21 nucleotides in length and possess a characteristic 2-nucleotide overhang at the 3' end. This unique structure allows for efficient loading into the RNA-induced silencing complex (RISC), which is responsible for target mRNA degradation.
The design of MISSION esiRNA involves selecting regions within the target gene that are highly conserved among different species. This ensures that the esiRNA can effectively silence the gene in a wide range of mammalian cells. Additionally, the selection process avoids regions with potential off-target effects, minimizing the risk of unintended gene silencing.
The MISSION esiRNA is available as a ready-to-use product, allowing researchers to easily perform large-scale RNAi screens. These screens involve the systematic knockdown of individual genes in a high-throughput manner, enabling the identification of genes involved in specific biological processes or disease pathways. By silencing genes of interest, researchers can observe the resulting phenotypic changes and gain insights into gene function and cellular processes.
The MISSION esiRNA is synthesized by in vitro transcription of long double-stranded RNA (dsRNA) templates, which are then processed by the endoribonuclease Dicer into small interfering RNAs (siRNAs). These siRNAs are approximately 21 nucleotides in length and possess a characteristic 2-nucleotide overhang at the 3' end. This unique structure allows for efficient loading into the RNA-induced silencing complex (RISC), which is responsible for target mRNA degradation.
The design of MISSION esiRNA involves selecting regions within the target gene that are highly conserved among different species. This ensures that the esiRNA can effectively silence the gene in a wide range of mammalian cells. Additionally, the selection process avoids regions with potential off-target effects, minimizing the risk of unintended gene silencing.
The MISSION esiRNA is available as a ready-to-use product, allowing researchers to easily perform large-scale RNAi screens. These screens involve the systematic knockdown of individual genes in a high-throughput manner, enabling the identification of genes involved in specific biological processes or disease pathways. By silencing genes of interest, researchers can observe the resulting phenotypic changes and gain insights into gene function and cellular processes.
3 هفته پیش
در تاریخ 1403/04/07 منتشر شده
است.
26
بـار بازدید شده