Acid Fast Staining Procedure

This video demonstrates the ACID FAST STAINING technique. Acid Fast  Staining Procedure is a differential staining procedure used to differentiate bacteria into acid-fast and non-acid-fast bacteria.
acid-fast bacteria have a waxy cell wall made from complex hydrocarbon known as mycolic acid. This difference in cell wall composition makes bacterial cells react differently to acid-fast stains.
After a successful Acid Fast staining procedure, Acid Fast bacteria appear red in color and non-acid Fast bacteria appear blue in color.

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About this video -
Friends in this video tutorial we are going to see how to do acid-fast staining  practical in animated demo video

Queries Solved:
1. How to perform acid Fast staining procedure
2. How to do acid-fast staining practical
3. How to stain bacteria in acid-fast staining.
4. How to make gram stain slide
5. How to do  acid-fast staining  in  biology lab techniques
6. How to stain tb bacteria


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0:00 Introduction
0:10 Acid Fast Staining Procedure
0:29 Mycobacterium Tuberculosis - Tuberculosis
2:38 Take a clean, grease-free Glass slide
2:57 Heat fixation of smear
3:40 Keep the slide on staining tray
3:44 Primary Stain: Carbol Fuchsin
5:08 Decoloriser : Acid-Alcohol Solution
6:07 Counterstain : Methylene Blue

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