Antibody-based tools and protocols for characterization of pluripotent stem cells
1.4 هزار بار بازدید -
10 سال پیش
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http://www.lifetechnologies.com/us/en...
Stem cell biology constitutes one of the fastest growing areas in the life sciences. Accordingly, there is strong demand for improving the characterization tools and protocols available to stem cell researchers. We have developed a series of antibody-based tool sets and protocols that facilitate detection of important cellular markers for pluripotent stem cells and the differentiated cell types that can be derived from them (e.g., three germ layers, neural stem cells, cardiomyocytes). A group of specific immunocytochemistry reagent sets were identified by screening panels of primary antibodies against established stem cell markers, and then matching them with appropriate dye-conjugated secondary antibodies. Optimized fixative and permeabilization solutions as well as blocking and wash buffers are also included in this kit. These reagents are versatile and offer a streamlined solution to acquire more information per sample via multiplex staining strategies that are compatible with a variety of imaging platforms, including the EVOS® FLoid and EVOS® FL imaging systems. We also developed live-cell imaging reagents to improve culture characterization and clone selection during the cellular reprogramming process for the derivation of new induced pluripotent stem cell lines. These live-cell imaging kits are composed of dye-conjugated primary antibodies against select cell surface markers that are paired together with an imaging medium specifically designed to maximize fluorescence signal detection while maintaining cell health. We anticipate that this series of protocol and technology improvements will significantly enhance the current characterization approaches available to stem cell researchers.
Stem cell biology constitutes one of the fastest growing areas in the life sciences. Accordingly, there is strong demand for improving the characterization tools and protocols available to stem cell researchers. We have developed a series of antibody-based tool sets and protocols that facilitate detection of important cellular markers for pluripotent stem cells and the differentiated cell types that can be derived from them (e.g., three germ layers, neural stem cells, cardiomyocytes). A group of specific immunocytochemistry reagent sets were identified by screening panels of primary antibodies against established stem cell markers, and then matching them with appropriate dye-conjugated secondary antibodies. Optimized fixative and permeabilization solutions as well as blocking and wash buffers are also included in this kit. These reagents are versatile and offer a streamlined solution to acquire more information per sample via multiplex staining strategies that are compatible with a variety of imaging platforms, including the EVOS® FLoid and EVOS® FL imaging systems. We also developed live-cell imaging reagents to improve culture characterization and clone selection during the cellular reprogramming process for the derivation of new induced pluripotent stem cell lines. These live-cell imaging kits are composed of dye-conjugated primary antibodies against select cell surface markers that are paired together with an imaging medium specifically designed to maximize fluorescence signal detection while maintaining cell health. We anticipate that this series of protocol and technology improvements will significantly enhance the current characterization approaches available to stem cell researchers.
10 سال پیش
در تاریخ 1393/08/26 منتشر شده
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