9. Protein Electrophoresis and Free Light Chains - Dr Bettina Chale-Matsau

The lecture will cover laboratory aspects of serum protein electrophoresis and serum free light chains. Serum protein electrophoresis is a powerful tool used in the investigation of patients suspected of monoclonal gammopathy and other protein disorders. It separates proteins based on their physical properties.  Abnormal proliferation of plasma cells is demonstrated by a monoclonal protein (M-protein), which can be identified using immunofixation (IFE). Serum free light chain (SFLC) testing methods have increased sensitivity compared to SPE and IFE. SFLC analysis provides an added advantage to identify patients that may be missed by SPE such as light chain multiple myeloma, amyloidosis and nonsecretory multiple myeloma.