Nuclei Isolation from Whole Tissue using Detergent & Enzyme Free Method
1.9 هزار بار بازدید -
2 سال پیش
-
Nuclei Isolation from Whole Tissue
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method - Experimental Protocol
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Single nuclei isolation relies on dissociation and detergent-based permeabilization of the cell membrane, steps that need optimization and are prone to introducing technical artifacts. We demonstrate a detergent and enzyme-free protocol for rapid isolation of intact nuclei directly from whole tissue, yielding nuclei suitable for single-nucleus RNA-seq (snRNA-Seq) or ATAC-seq.
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Watch the full video at https://www.jove.com/v/61471/nuclei-i..., no sign up needed for open access content, or request a trial to get instant access to our full library.
Single nuclei isolation relies on dissociation and detergent-based permeabilization of the cell membrane, steps that need optimization and are prone to introducing technical artifacts. We demonstrate a detergent and enzyme-free protocol for rapid isolation of intact nuclei directly from whole tissue, yielding nuclei suitable for single-nucleus RNA-seq (snRNA-Seq) or ATAC-seq.
Visit https://www.jove.com/?utm_source=yout... to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard and the University of Cambridge, benefit from using JoVE’s extensive library of 14000 videos in their research and teaching.
Follow JoVE on Twitter: Twitter: JoVEJournal
Like JoVE on Facebook: Facebook: JOVEjournal
Subscribe to our channel: jovejournalofvisualizedexperiments
2 سال پیش
در تاریخ 1400/11/30 منتشر شده
است.
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