Western blotting technique | Detection of proteins | Principle | Procedure | Bio science

Western blotting technique | Detection of proteins | Principle | Procedure | Bio science

Western blotting (immunoblotting, protein blotting) is a sensitive immunological method for detecting electrophoretically separated proteins. The technique was developed independently by several groups in 1979 and was later termed ‘western blotting’ because of its analogy to Southern and northern blotting. Its characteristic feature is the transfer of a complex protein pattern resolved by slab gel electrophoresis to a membrane which serves as a carrier for all subsequent immunochemical reactions. Major fields of application in basic research are determination of molecular weights of protein antigens, detection of antigens in crude mixtures of proteins, and assessment of the quality of animal antisera. Western blotting has found important clinical applications in autoimmune disease, allergy, infectious diseases and others.

Check this playlist for other videos on immumotechnology :-
Immunotechnology

Follow us on Instagram:-
https://www.instagram.com/invites/con...

Subscribe to my channel for more other videos :-
@bioscience6853

Checkout other playlists for other more videos :-

BIOTECHNOLOGY :-
BIOTECHNOLOGY

PLANT BIOTECHNOLOY :-
Plant biotechnology

rDNA TECHNOLOGY :-
r DNA Technology

IMMUNOLOGY :-
Immunology

IMMUNOTECHNOLOGY :-
Immunotechnology

BOTANY :-
BOTANY

ZOOLOGY :-
ZOOLOGY

ANIMAL BIOTECHNOLOY :-
Animal biotechnology

CARBOHYDRATES | BIOCHEMISTRY :-
CARBOHYDRATES || BIOCHEMISTRY

FERMENTATION :-
FERMENTATION

BIOSTATISTICS :-
BIOSTATISTICS

GENETICS :-
GENETICS

For other more playlists and videos, checkout my channel :-
@bioscience6853

#sdspage #antigens #antibodies #immunology #immunotechnology
immunoblotting
Western blotting technique
Western blotting procedure
Western blot detection
Western blotting shomu
Western blotting detection
protein blotting
Southern blotting
Northern blotting
sds page
Western blotting principle
Alampally Phanindra
Phanindra gupta
Protein blotting
western blotting technique
western blotting step by step
western blotting
western blot
blotting techniques
western blot test
western blot test in hindi

• #people_blogs
• #immunoblotting
• #western_blotting_technique
• #western_blotting_procedure
• #western_blot_detection
• #western_blotting_shomu
• #western_blotting_detection
• #protein_blotting
• #southern_blotting
• #northern_blotting
• #sds_page
• #western_blotting_principle
• #alampally_phanindra
• #phanindra_gupta
• #protein_blotting
• #western_blotting_technique
• #western_blotting_step_by_step
• #western_blotting
• #western_blot
• #blotting_techniques
• #western_blot_test
• #western_blot_test_in_hindi