Indirect coomb test procedure / ICT test procedure
30.5 هزار بار بازدید -
2 سال پیش
-
Indirec coomb test (ICT)Label three
Indirec coomb test (ICT)
Label three test tubes as T (test serum) PC (Positive control) and NC (negative control).
In the tube labeled as T (Test), take 2 drops of test serum.
In the test tube labeled as PC (Positive control), take 1 drop of anti D serum.
In the test tube labeled as NC (Negative control), take 1 drop of normal saline.
Add one drop of 5 % saline suspension of the pooled ‘O’ Rho (D) positive cells in each tube.
Incubate all the three tubes for one hour at 37°C.
Wash the cells three times in normal saline to remove excess serum with no free antibodies, (in the case of inadequate washings of the red cells, negative results may be obtained).
Add two drops of Coombs serum (anti human serum) to each tube.
Keep for 5 minutes and then centrifuge at 1,500 RPM for one minute.
Resuspend the cells and examine macroscopically as well as microscopically.
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#Icttest
#indirectcoombtestprocedure
#coombtestprocedure
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Label three test tubes as T (test serum) PC (Positive control) and NC (negative control).
In the tube labeled as T (Test), take 2 drops of test serum.
In the test tube labeled as PC (Positive control), take 1 drop of anti D serum.
In the test tube labeled as NC (Negative control), take 1 drop of normal saline.
Add one drop of 5 % saline suspension of the pooled ‘O’ Rho (D) positive cells in each tube.
Incubate all the three tubes for one hour at 37°C.
Wash the cells three times in normal saline to remove excess serum with no free antibodies, (in the case of inadequate washings of the red cells, negative results may be obtained).
Add two drops of Coombs serum (anti human serum) to each tube.
Keep for 5 minutes and then centrifuge at 1,500 RPM for one minute.
Resuspend the cells and examine macroscopically as well as microscopically.
#indirectcoombtest
#Icttest
#indirectcoombtestprocedure
#coombtestprocedure
#Indirectcoombtestkesekre
#manojmlt
2 سال پیش
در تاریخ 1400/12/17 منتشر شده
است.
30,566
بـار بازدید شده