Indirect Coombs Test Procedure | Indirect Antiglobulin Test (IAT-Step by Step)
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This video is about Indirect
This video is about Indirect Coombs Test Procedure | Indirect Antiglobulin Test (IAT-Step by Step)
Indirect Antiglobulin Test(IAT)
00:00 Intro
00:13Take 3 test tubes and label them as S1, S2, S3.
00:38 Add 1 drop of S1 into the tube marked as S1.
00:55 Add 1 drop of S2 into the tube marked as S2.
01:09 Add 1 drop of S3 into the tube marked as S3.
01:21 Add 2 drops of test serum.
-Add 2 drops of LISS reagent.
-Mix well.
01:50 Keep them at 37'C,12-15 minutes.
02:17 After the incubation, wash the cells in normal saline to remove excess serum. (4000 rpm,1min.)
06:29 Add 2 drops of Coomb's reagent into each tube.
06:53 Centrifuge the tubes at 1000 rpm for 1 minute.
07:40 Resuspend the cells by gentle agitation and examine microscopically or microscopically for agglutination.
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Indirect Antiglobulin Test(IAT)
00:00 Intro
00:13Take 3 test tubes and label them as S1, S2, S3.
00:38 Add 1 drop of S1 into the tube marked as S1.
00:55 Add 1 drop of S2 into the tube marked as S2.
01:09 Add 1 drop of S3 into the tube marked as S3.
01:21 Add 2 drops of test serum.
-Add 2 drops of LISS reagent.
-Mix well.
01:50 Keep them at 37'C,12-15 minutes.
02:17 After the incubation, wash the cells in normal saline to remove excess serum. (4000 rpm,1min.)
06:29 Add 2 drops of Coomb's reagent into each tube.
06:53 Centrifuge the tubes at 1000 rpm for 1 minute.
07:40 Resuspend the cells by gentle agitation and examine microscopically or microscopically for agglutination.
For more information visit:- https://medilabzone.com
👉FB Page:- Facebook: Medilabzone
#coombstest #indirect #hematology #antiglobulin_test
If you like my channel
like
share
comment
subscribe to my channel
3 سال پیش
در تاریخ 1400/08/13 منتشر شده
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