PCR Cloning (III)- Ligation

( http://www.abnova.com ) - DNA ligation joins two linear DNA fragments together with covalent phosphodiester bonds between 3' hydroxyl ends of one nucleotide with the 5' phosphate end of another. In PCR cloning, insert DNA and vector digested by the same restriction enzyme can be ligated by DNA ligase. More videos at Abnova http://www.abnova.com

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