The Agar Sandwich Method | Eliminating Bacterial Contamination from a Mushroom Culture
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The agar sandwich method is
The agar sandwich method is a great way to remove bacteria from a mushroom culture on agar plates. The method is pretty straight forward, just pour warm sterilized agar overtop of the contaminated agar culture you are trying to clean. The mycelium will grow through the top layer of agar but the bacteria will stay in the middle layer. This leaves us the opportunity to transfer the surface mycelium to a new plate ensuring the removal of bacteria.
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Mushroom liquid culture and supplies: https://sporensprout.etsy.com
🍄Check out my social links! https://linktr.ee/sporensprout
Recommended supplies:
Nutrient agar powder 100 grams: https://amzn.to/4bBk44E
Media bottles: https://amzn.to/49zS3cd
Presto pressure cooker: https://amzn.to/3HW3v5T
Digital scale: https://amzn.to/48lwbjO
Parafilm: https://amzn.to/3HTO3aw
20 pack sterile Petri dishes: https://amzn.to/3UC7agR
Premade agar plates: https://amzn.to/48dqB2w
*Clicking my affiliate links and making a purchase may result in me earning a small commission, thank you for supporting the channel!
Join the new mushroom cultivation discord community! Discord: discord
Join the new exclusive member channel to get access to perks:
@sporensprout
#growyourown #mushrooms
5 ماه پیش
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