# 1 Most Common Problem in HPLC: Air Bubble in the Pump

The most common issue encountered in High-Performance Liquid Chromatography (HPLC) - an air bubble in the pump. Discover expert tips and techniques from Axion Labs to quickly and effectively resolve this problem.

Key Highlights:

Identifying Air Bubbles: Learn to recognize the symptoms of an air bubble in your HPLC pump, such as unusual pressure ripple and fluctuating UV signals​​.

Simple Fixing Method: We demonstrate a straightforward procedure to eliminate air bubbles, including opening the purge valve and adjusting the flow rate​​.

Verifying Solution Effectiveness: Understand how to check if the air bubble has been successfully removed by observing the pressure ripple and ensuring it falls within the acceptable range​​.

Software Tips: Gain insights into using diagnostic tools in HPLC software to monitor and confirm the pressure ripple status​​.


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Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC skills with our 5 day hands-on LC/GC bootcamp. If you can't make it to Chicago choose one of our online courses. After taking the LC/GC bootcamp further advance your skills by taking the advanced topics courses.

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