Processes of Recombinant DNA Technology | Class 12 Biology Chapter 11 (2022-23)

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✅ In this video,

✔️ Class: 12th
✔️ Subject: Biology
✔️ Chapter: Biotechnology: Principles and Processes (Chapter 11)
✔️ Topic Name: Processes of Recombinant DNA Technology
✔️ Topics Covered in This Video (By Shipra Mam): Recombinant DNA Technology, DNA Isolation, Fragmentation of DNA by Restriction Enzyme, Endonuclease, Exonuclease, Fragment Detection, Gel Electrophoresis, Separation and Isolation of a Desired DNA Fragment, Amplification of Gene of Interest Using PCR, Ligation of the DNA Fragment into a Vector, Transferring the Recombinant DNA into the Host, Production of the Host Cells in a Nutrient Medium at a large scale
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00:00 Introduction
01:17 Process of Recombinant DNA Technology
07:10 Steps Involved in Recombinant DNA Technology
18:20 Isolation of the Genetic Material
19:41 Fragmentation of DNA by RE
36:21 Separation & Isolation of a Desired DNA Fragment
46:18 Amplification of Gene of Interest Using PCR
48:41 Ligation of the DNA Fragment Into a Vector
54:57 Transferring the Recombinant DNA Into the Host
1:07:26 Production of the Host Cells in a Nutrient Medium at a Large Scale
1:13:07 NCERT Theory
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