How to Perform Total Protein Normalization for Western Blot Protein Analysis with Phoretix 1D

Learn how to normalize your western blot signal to your total protein using TotalLab's Phoretix 1D image analysis software.

In this video, I'm going to be guiding you through the various normalization algorithms (single channel, housekeeping protein/gene or total protein) available to you within Phoretix 1D with a specific focus on how to perform total protein normalization as an industry standard.

00:13 - Intro
01:12 - Single band normalization
01:24 - Setting reference bands per channel
02:25 - Normalizing to a housekeeping protein or gene
03:03 - Total protein Normalization
03:40 - How total protein normalization is calculated
05:03 - How to Get a Free Trial of Phoretix 1D

Phoretix 1D is also available in a fully 21 CFR Part 11 and EU Annex 11 compliant version for use in regulated industries such as medical device production.

For a free trial of Phoretix 1D to see how well it works with your images, please fill in the form here:
https://totallab.com/products/phoreti...

Phoretix 1D can provide quantitative analysis of blot and gel images, lateral flow test images, TLC (thin layer chromotography) images and many more.

See our other image analysis-based products here: https://totallab.com/products/

And if you want to get in touch with us here at TotalLab:

Website: http://www.totallab.com/
LinkedIn: LinkedIn: totallab-ltd

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