SDS-PAGE theory & practice: into the buffer and behind the scenes!

Today I ran my 739th SDS-PAGE since I started counting, and I videoed & talked it out for you and added it to my more theory-y talk so you get theory + practical for the price of a free video! SDS-PAGE is a technique we use to separate proteins by size (technically length) by unfolding them, coating them in a detergent (artificial soap) called SDS (Sodium DoDecylSulfate), and sending them traveling through a PolyAcrylamide Gel mesh using Electrophoresis). It's super duper useful, so let's talk about it

And if you want a written & graphics version: http://bit.ly/sdspageruler