Quantitative Polymerase Chain Reaction (qPCR)

Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 Full protocols for all assays available at ahmed-lab.org This video provides an overview of the theory and practice of quantitative PCR, a widely used technique in molecular biology laboratories in order to measure the mRNA expression of genes or to measure activation of gene transcription for specific genes. Here, we go through both the theory behind it and the practical aspects of getting primers, designing them appropriately, planning out your experiment, and interpreting and troubleshooting results. This work is coming from the Ahmed Lab at Northwestern supported by funding from the NIH/NINDS.