Immunopharmacology (Part-12) Monoclonal Antibodies (01) = Introduction and Production Techniques.
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An antibody is a protein produced by the body's immune system in response to antigens, which are harmful substances. Antigens include bacteria, fungi, parasites, viruses, chemicals, and other substances the immune system identifies as foreign. Sometimes the body mistakenly identifies normal tissues as foreign and produces antibodies against the tissue. This is the underlying cause of autoimmune conditions such as rheumatoid arthritis and multiple sclerosis or MS.
Antibodies are naturally produced by the immune system. However, scientists can produce antibodies in the lab that mimic the action of the immune system. These man-made (synthetic) antibodies act against proteins that attack normal tissues in people with autoimmune disorders. Man-made antibodies are produced by introducing human genes that produce antibodies into mice or other suitable mammal. The mice than are vaccinated with the antigen that scientists want to produce antibodies against. This causes the immune cells of the mice to produce the desired human antibody. The term monoclonal antibody means that the man-made antibody is synthesized from cloned immune cells, and the identical monoclonal antibody produced binds to one type of antigen. Polyclonal antibodies are synthesized from different immune cells and the antibodies produced to bind to multiple antigens.
Steps -
(1) Immunization = Inject antigen in mouse
(2) Screen ing of antibody production and removal of spleen when antibody levels are high
(3) The fusion of myeloma cells with spleen cells using polyethene glycol to produce hybridoma cells
(4) Selection of hybridoma cells by culturing in HAT media
(5) Checking the hybridoma cells and subculturing them
(6) Cloning of hybridoma cell
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Download "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Test and other GPAT Materials - https://play.google.com/store/apps/de...
An antibody is a protein produced by the body's immune system in response to antigens, which are harmful substances. Antigens include bacteria, fungi, parasites, viruses, chemicals, and other substances the immune system identifies as foreign. Sometimes the body mistakenly identifies normal tissues as foreign and produces antibodies against the tissue. This is the underlying cause of autoimmune conditions such as rheumatoid arthritis and multiple sclerosis or MS.
Antibodies are naturally produced by the immune system. However, scientists can produce antibodies in the lab that mimic the action of the immune system. These man-made (synthetic) antibodies act against proteins that attack normal tissues in people with autoimmune disorders. Man-made antibodies are produced by introducing human genes that produce antibodies into mice or other suitable mammal. The mice than are vaccinated with the antigen that scientists want to produce antibodies against. This causes the immune cells of the mice to produce the desired human antibody. The term monoclonal antibody means that the man-made antibody is synthesized from cloned immune cells, and the identical monoclonal antibody produced binds to one type of antigen. Polyclonal antibodies are synthesized from different immune cells and the antibodies produced to bind to multiple antigens.
Steps -
(1) Immunization = Inject antigen in mouse
(2) Screen ing of antibody production and removal of spleen when antibody levels are high
(3) The fusion of myeloma cells with spleen cells using polyethene glycol to produce hybridoma cells
(4) Selection of hybridoma cells by culturing in HAT media
(5) Checking the hybridoma cells and subculturing them
(6) Cloning of hybridoma cell
Get in touch with the solution by just clicking following links-
Facebook Group- Facebook: solutionpharamcy
Facebook Page- Facebook: pharmavideo
New channel (Pharmacy Dictionary) - @pharmacydictionary
Instagram- Instagram: solutionpharmacy
E-Mail for official and other work - [email protected]
LinkedIn- LinkedIn: pushpendrakpatel
#solutionpharmacy #Pharmacologyclass #GPATonlinetest
4 سال پیش
در تاریخ 1399/06/17 منتشر شده
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