Patch Clamp Explained (Cell-Attached, Whole Cell, Inside Out, Outside Out Configurations) | Clip

Welcome to Science With Tal!

In this clip of the Signal Propagation in the Neuron video, we will discuss the different configurations of the patch clamp (cell-attached, whole cell, inside out, outside out configurations). We will then use the outside out configuration on a leak ionic channel to see how the technique can help us. To improve the quality of my content, I highly value the feedback from the viewer so do not hesitate to give any feedback in the comment section.

Timestamps

0:00 Introduction
0:17 Motivation for the patch clamp
0:38 Cell attached patch clamp
1:55 Inside out patch clamp
2:15 Whole cell patch clamp
2:34 Outside out patch clamp
3:00 Summary of 4 configurations
3:11 IV curve from patch clamp (Leak potassium channel)
5:38 Word on voltage gated channels
6:47 Conclusion

Resources used

Here is a list of the resources that I’ve used to produce this video. (Author: title resource)

Dale Purves: Neuroscience (6th edition)
Eric Kandel: Principles of neural science (6th edition)
Alan Hodgkin & Andrew Huxley: A quantitative description of membrane current and its application to conduction and excitation in nerve
Alan Hodgkin & William Albert Hugh Rushton: The electrical constants of a crustacean nerve fibre.

To have more information on these resources, you can refer to the conclusion section where a more formal citation is provided.

Video credits

Writing: Tal Klimenko
Voice: Tal Klimenko
Animations: Tal Klimenko
Drawings: Tal Klimenko
Editing: Tal Klimenko
Introductory jingle: Thierry Du Sablond
Conclusion music: lukrembo - jay (lukrembo - jay (royalty free vlog music))