For how much time we can store CTAB buffer ? #youtubeshorts #shorts #biotechnology
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CTAB buffer is a commonly
CTAB buffer is a commonly used buffer for DNA extraction from various sources such as plants and fungi. The stability of CTAB buffer can depend on factors such as storage conditions and the purity of the buffer components. Generally, CTAB buffer can be stored for several months to a year at temp ranging from 4°C to 30°C , and for longer periods at -20°C or -80°C.
However, it is important to note that the quality of the extracted DNA may be affected by the storage time and conditions of the CTAB buffer. The buffer components may degrade over time, leading to reduced efficiency of DNA extraction or even DNA degradation. It is recommended to use freshly prepared CTAB buffer whenever possible to ensure optimal DNA yield and quality. If it is necessary to store CTAB buffer for an extended period, it is advisable to aliquot it into small portions and store them at -20°C or -80°C to minimize repeated freeze-thaw cycles.
However, it is important to note that the quality of the extracted DNA may be affected by the storage time and conditions of the CTAB buffer. The buffer components may degrade over time, leading to reduced efficiency of DNA extraction or even DNA degradation. It is recommended to use freshly prepared CTAB buffer whenever possible to ensure optimal DNA yield and quality. If it is necessary to store CTAB buffer for an extended period, it is advisable to aliquot it into small portions and store them at -20°C or -80°C to minimize repeated freeze-thaw cycles.
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در تاریخ 1402/02/01 منتشر شده
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