Basic adjustments of fluorescent images with FIJI

Recommended: watch this video in HD for better text rendering.

00:00 Introduction
00:23 FIJI Installation
03:25 FIJI Updates
05:21 Opening fluorescence microscopy images
6:35 FIJI Bio-Formats Import options
9:00 Information in image title bar
10:22 Changing channel and Z planes in multidimensional datasets
11:19 Opening Brightness/Contrast and Channels tool
12:19 Channels tool: grayscale view, selecting display colors, composite view
15:50 Microscopy data is numbers associated with pixels that get displayed in different ways
20:37 Brightness/Contrast tool: adjusting how channels look
27:41 Getting images out of FIJI that are usable in other software
30:10 Adding a scale bar
34:03 Exporting individual channels
34:51 Putting everything together in a presentation
36:14 Working with more than one image: tile displayed windows, synchronize windows
39:49 Conditions for properly comparing multiple images
42:15 Propagate display settings across multiple images
45:31 Opening images from Zeiss microscopes in Zeiss software to easily access metadata
48:48 Guidelines for writing proper materials and methods: https://www.med.unc.edu/microscopy/ri...
49:07 Acknowledge the Microscopy Services Laboratory: https://www.med.unc.edu/microscopy/us...