Manual platelet count - hemocytometer Procedure | Calculation | interpretation
15.8 هزار بار بازدید -
12 ماه پیش
-
Manual platelet count - hemocytometer
Manual platelet count - hemocytometer Procedure | Calculation | interpretation
Step 1: Sample Collection
"First, we'll need a small blood sample, about 20 microliters.
Step 2: Ammonium Oxalate Treatment
"Next, we add 1.8 milliliters of a 1% ammonium oxalate solution to the sample. This solution will lyse the red and white blood cells while leaving the platelets intact."
Step 3: Waiting for Lysis
"Now, we let the sample sit for 15 minutes to ensure complete lysis of the red blood cells."
Step 4: Preparing the Neubauer Chamber
"While we wait, let's prepare our counting chamber. We're using a Neubauer chamber for this."
Step 5: Equilibration
"To prevent drying and ensure even cell distribution, we leave the prepared Neubauer chamber in a high-humidity environment for another 15 minutes."
Step 6: Platelet Counting
"Time to count the platelets! Under a microscope, we focus on the central square labeled 'P.'"
Step 7: Calculating the Platelet Count
"Now, for the math. We count the platelets in that central square and use the formula to calculate the platelet count per microliter."
Step 8: Recording and Reporting
"Don't forget to record your results accurately in your lab log and report them following your lab's guidelines."
medilablife,platelet count calculation in slide,manual platelet count normal range,manual platelet count formula dog,manual platelet count microscope,manual platelet count vs automated platelet count,how to calculate platelet count,manual platelet count formula veterinary,manual platelet count formula cat,platelet count manual method,platelet count high,platelet count,platelet count manual method procedure,Platelets under microscope,platelets on smear
Step 1: Sample Collection
"First, we'll need a small blood sample, about 20 microliters.
Step 2: Ammonium Oxalate Treatment
"Next, we add 1.8 milliliters of a 1% ammonium oxalate solution to the sample. This solution will lyse the red and white blood cells while leaving the platelets intact."
Step 3: Waiting for Lysis
"Now, we let the sample sit for 15 minutes to ensure complete lysis of the red blood cells."
Step 4: Preparing the Neubauer Chamber
"While we wait, let's prepare our counting chamber. We're using a Neubauer chamber for this."
Step 5: Equilibration
"To prevent drying and ensure even cell distribution, we leave the prepared Neubauer chamber in a high-humidity environment for another 15 minutes."
Step 6: Platelet Counting
"Time to count the platelets! Under a microscope, we focus on the central square labeled 'P.'"
Step 7: Calculating the Platelet Count
"Now, for the math. We count the platelets in that central square and use the formula to calculate the platelet count per microliter."
Step 8: Recording and Reporting
"Don't forget to record your results accurately in your lab log and report them following your lab's guidelines."
medilablife,platelet count calculation in slide,manual platelet count normal range,manual platelet count formula dog,manual platelet count microscope,manual platelet count vs automated platelet count,how to calculate platelet count,manual platelet count formula veterinary,manual platelet count formula cat,platelet count manual method,platelet count high,platelet count,platelet count manual method procedure,Platelets under microscope,platelets on smear
12 ماه پیش
در تاریخ 1402/06/29 منتشر شده
است.
15,883
بـار بازدید شده