Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) l Protocol Preview
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2 سال پیش
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Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP - a 2 minute Preview of the Experimental Protocol
Kun-Yong Kim, Eriona Hysolli, In-Hyun Park
Yale School of Medicine, Yale Stem Cell Center, Department of Genetics;
A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.
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Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP - a 2 minute Preview of the Experimental Protocol
Kun-Yong Kim, Eriona Hysolli, In-Hyun Park
Yale School of Medicine, Yale Stem Cell Center, Department of Genetics;
A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.
Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: jovejournalofvisualizedexperiments
2 سال پیش
در تاریخ 1401/04/02 منتشر شده
است.
609
بـار بازدید شده