DIY Scanning Laser Microscope
433.7 هزار بار بازدید -
3 سال پیش
-
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I built a 3D printed, scanning laser confocal microscope so I could collect 3D surface topology and profilometry data! The microscope stage is based on an OpenFlexure "Delta" stage, and the confocal optics are constructed with a 3D printed body, some lenses, a 405nm laser, pinhole and photodiode. The microscope is controlled by a Raspberry Pi (which controls an arduino to move the steppers).
It's not beautiful, it takes forever to scan, and the images are of dubious quality... but it works!
OpenFlexure: https://openflexure.org/
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Intro
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Confocal vs Widefield Microscopy
https://www.seevid.ir/fa/w/9TYlQ4urcg8 OpenFlexure Motion Platform
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Confocal optical breakdown
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Delta motion stage
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Photodiode amplifier
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Confocal Pinhole demonstration
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Camera vs Photodiode
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Data processing considerations
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Images and results!
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Optimizations
https://www.seevid.ir/fa/w/9TYlQ4urcg8 Discord! Come hang out with us!
3 سال پیش
در تاریخ 1400/02/01 منتشر شده
است.
433,700
بـار بازدید شده