Do PCR Better than Gradient - A Stop-motion Animation

Over 6,000 PCR-tubes and 30 plates were used to make this stop-motion animation. The video imagines a day with gradients, and how the Veriti™ Thermal Cycler allows you to do PCR better than gradient. See the "Making Of" video here:    • Making Of: Do PCR Better than Gradien...   With the Veriflex™ technology you can independently set each of the 6 individual zones rather than just set the high and low temperatures of a gradient. This means that optimization of a new reaction can be done using 6 precise temperatures, (giving the user more freedom to try varying annealing temperatures) and you can effectively run up to 6 separate thermal cycling protocols on the same instrument at the same time. For more information, go to www.thermofisher.com/veriti [audio transcript] So I'm doing gradient PCR, trying to optimize the annealing, and I need to find the precise temperature. But instead of setting a range, I wish I could set specific temperatures at the same time. And then I get to imagining: Imagining a day with gradients and no precision. What it would that be like? It would be like: In the middle of my dreamy slumber of my paper being published in Cell, I'm woken - and I don't know whether I overslept or not - because my alarm clock can't be set at a precise time! And then I get in the shower - and try to find the right mix of hot and cold, but I'm just not able to get the temperature right! It would be like: Making my breakfast. And yet no matter how hard I try, I just can't get the perfect toast! It would be like: Heading off for a much needed coffee at my favorite coffee shop, and I'm not able to order the right size! So with a coffee so ridiculously big it needs a straw, I head for my car. I get in. Take off. But I have only a vague idea how fast I'm going! Then I find a place to park. Pull up. But again there's this gradient thing going on, and I can't be precise about of time I want to spend here! It would be like: Getting in the lift and I'm just not able to find the right floor! So imagine if I could actually set the lift to 6 exact floors all at the same time??! And that's precisely what I want to do with my PCR! Instead of setting the high and low temperatures of a gradient, now I would actually be able to set a number of precise temperatures! Well : WELCOME to a Life BETTER THAN GRADIENT! Veriti™ Thermal Cycler with Veriflex™ Blocks - 6 temperature blocks - Set individual temperatures for each block - 6 accurate temperatures for better PCR optimization - Combine up to 6 different conditions in one PCR run - Precise incubations for non-PCR workflows For more information, or to request a demonstration of the Veriti™ Thermal Cycler in your lab, please contact your Thermo Fisher Scientific representative or complete the Contact Me form at www.thermofisher.com/veriti.

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