Extracellular Vesicles as Communicators Between Cells - A Role in Cancer Dissemination

Presented By: Laurence Blavier Sarte, PhD Speaker Biography: Laurence Blavier Sarte earned her M.S. and Ph.D. in Cell Biology from the de Duve Institute at the Catholic University of Leuven, Belgium, where she investigated the role of Matrix Metalloproteinases (MMPs) in the development and remodeling of embryonic long bones. She joined the laboratory of Dr. Yves DeClerck at Children’s Hospital Los Angeles in the division of Hematology-Oncology, studying the role of MMPs and their inhibitors (TIMPs) in epithelial-mesenchymal transition and cancer progression. She branched out and did some research at the National Institute of Health studying the MMPs in joint diseases. She returned to CHLA and is currently a Staff Scientist with over 25 years of research experience in developmental and cancer biology. Her research has been focused on the interaction of cancer cells with their tumor microenvironment. Most recently she is investigating the role of tumor-derived extracellular vesicles in the pre-metastatic niche. She is a lecturer at the University of Southern California contributing to the Graduate Biology Course on Cancer Cells in their Social Environment with an emphasis on EVs in cancer progression and as a diagnostic and therapeutic tool. Webinar: Extracellular Vesicles as Communicators Between Cells - A Role in Cancer Dissemination Webinar Abstract: The capture of tumor-derived extracellular vesicles (TEVs) by cells in the tumor microenvironment (TME) contributes to metastasis and notably to the formation of the pre-metastatic niche (PMN). However, due to the challenges associated with modelling release of small EVs in vivo, the kinetics of PMN formation in response to endogenously released TEVs have not been examined. Here, we have studied the endogenous release of TEVs in mice orthotopically implanted with metastatic human melanoma (MEL) and neuroblastoma (NB) cells releasing GFP-tagged EVs (GFTEVs) and their capture by host cells to demonstrate the active contribution of TEVs to metastasis. Human GFTEVs captured by mouse macrophages in vitro resulted in transfer of GFP vesicles and the human exosomal miR-1246. Mice orthotopically implanted with MEL or NB cells showed the presence of TEVs in the blood between 5 and 28 days after implantation.... Earn PACE Credits: 1. Make sure you’re a registered member of Labroots (www.labroots.com/ms/webinar/extracellular-vesicles…) 2. Watch the webinar on YouTube or on the Labroots Website (www.labroots.com/ms/webinar/extracellular-vesicles…) 3. Click Here to get your PACE credits (Expiration date – September 20, 2025) (www.labroots.com/credit/pace-credits/8360/cert) Labroots on Social: Facebook: www.facebook.com/LabRootsInc Twitter: twitter.com/LabRoots LinkedIn: www.linkedin.com/company/labroots Instagram: www.instagram.com/labrootsinc Pinterest: www.pinterest.com/labroots/ SnapChat: labroots_inc