Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
338 بار بازدید -
2 سال پیش
-
A comprehensive display of the
A comprehensive display of the technical aspects of Whole Genome Array Comparative Genomic Hybridization (CGH) is presented in this demonstration. This technique involves the comparison of DNA samples from different sources using microarray technology to identify genetic variations and alterations across the entire genome. By analyzing the hybridization patterns, researchers can gain valuable insights into genomic rearrangements, copy number variations, and other structural changes that may be associated with various diseases or conditions. This technical demonstration aims to showcase the intricate process and methodology behind Whole Genome Array CGH, highlighting its significance in advancing our understanding of the human genome and its implications in clinical research and diagnostics.
During this demonstration, the technical aspects of Whole Genome Array Comparative Genomic Hybridization (CGH) will be thoroughly explained and showcased. This technique involves the comparison of DNA samples from different sources using microarray technology, which allows for the identification of genetic variations and alterations across the entire genome.
The process begins with the collection of DNA samples from various sources, such as healthy individuals, patients with specific diseases, or individuals with known genetic abnormalities. These samples are then labeled with different fluorescent dyes, typically green and red, to distinguish between the two sources during the hybridization process.
Next, the labeled DNA samples are mixed together and applied to a microarray slide, which contains thousands or even millions of DNA probes that represent specific regions of the genome. These probes are designed to bind to complementary DNA sequences, allowing for the detection of genetic variations and alterations.
The hybridization process occurs when the labeled DNA samples bind to their complementary DNA probes on the microarray slide. This binding is facilitated by the specific base pairing between the DNA sequences. The slide is then washed to remove any unbound DNA, leaving only the hybridized DNA probes on the slide.
After the hybridization process, the microarray slide is scanned using a specialized scanner that detects the fluorescent signals emitted by the labeled DNA samples. The scanner captures the intensity of the green and red signals, which correspond to the abundance of DNA from each source.
By analyzing the hybridization patterns, researchers can gain valuable insights into genomic rearrangements, copy number variations, and other structural changes that may be associated with various diseases or conditions. For example, an increased intensity of the red signal compared to the green signal may indicate a duplication or amplification of a specific DNA region in the patient sample.
During this demonstration, the technical aspects of Whole Genome Array Comparative Genomic Hybridization (CGH) will be thoroughly explained and showcased. This technique involves the comparison of DNA samples from different sources using microarray technology, which allows for the identification of genetic variations and alterations across the entire genome.
The process begins with the collection of DNA samples from various sources, such as healthy individuals, patients with specific diseases, or individuals with known genetic abnormalities. These samples are then labeled with different fluorescent dyes, typically green and red, to distinguish between the two sources during the hybridization process.
Next, the labeled DNA samples are mixed together and applied to a microarray slide, which contains thousands or even millions of DNA probes that represent specific regions of the genome. These probes are designed to bind to complementary DNA sequences, allowing for the detection of genetic variations and alterations.
The hybridization process occurs when the labeled DNA samples bind to their complementary DNA probes on the microarray slide. This binding is facilitated by the specific base pairing between the DNA sequences. The slide is then washed to remove any unbound DNA, leaving only the hybridized DNA probes on the slide.
After the hybridization process, the microarray slide is scanned using a specialized scanner that detects the fluorescent signals emitted by the labeled DNA samples. The scanner captures the intensity of the green and red signals, which correspond to the abundance of DNA from each source.
By analyzing the hybridization patterns, researchers can gain valuable insights into genomic rearrangements, copy number variations, and other structural changes that may be associated with various diseases or conditions. For example, an increased intensity of the red signal compared to the green signal may indicate a duplication or amplification of a specific DNA region in the patient sample.
2 سال پیش
در تاریخ 1401/11/24 منتشر شده
است.
338
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