(07) Instrumentation of UV visible Spectroscopy | Instrumental Methods of Analysis | B.Pharmacy 7th
2.5 هزار بار بازدید -
7 ماه پیش
-
Download the "Solution Pharmacy" Mobile
Download the "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Tests and other GPAT Materials - play.google.com/store/apps/details?id=co.bolton.nq…
UV-visible spectroscopy involves specific instrumentation designed to measure the absorption of light in the ultraviolet and visible regions of the electromagnetic spectrum. The basic components of a UV-visible spectrophotometer include:
1. Light Source:
a. Tungsten-Halogen Lamp (for visible region): Emits light in the visible range (approximately 350-800 nm).
b. Deuterium Lamp (for UV region): Emits UV light (190-350 nm). Sometimes, Xenon lamps are also used for broader UV coverage.
2. Monochromator:
a. Entrance Slit: Allows light to enter the monochromator.
b. Prism or Grating: Disperses the light into its component wavelengths.
c. Exit Slit: Selects the desired wavelength to pass through to the sample.
3. Sample Compartment:
a. Cuvettes or Sample Holder: Hold the sample solution for analysis. Cuvettes are typically made of quartz or glass and come in various path lengths (1 cm being standard).
4. Temperature Control: Some instruments include temperature-controlled sample compartments for studying temperature-dependent reactions.
5. Detector:
Photodetector (Photomultiplier Tube or Photodiode Array): Measures the intensity of light transmitted through the sample at the selected wavelength.
6. Data Processing and Display:
a. Microprocessor and Software: Control the instrument, collect data, and perform calculations.
b. Display Screen: Shows the absorption spectrum and other relevant data.
c. Output Devices: Data can be printed or exported to external devices for further analysis.
7. Operation:
1. The light from the source passes through the monochromator, which selects the desired wavelength.
2. The monochromatic light then passes through the sample in the cuvette or sample holder.
3. The photodetector measures the intensity of transmitted light.
4. The instrument calculates the absorbance using the Beer-Lambert Law and displays the absorption spectrum.
Some Advanced Features:
1. Scanning Speed: Some instruments offer high scanning speeds for rapid data acquisition.
2. Wavelength Accuracy: Precise wavelength control and calibration are crucial for accurate measurements.
3. Range and Resolution: Different instruments offer various ranges and resolutions based on the application.
4. Automation and Connectivity: Automation features for multiple sample analysis and connectivity options for data transfer or integration with other instruments or software.
Practical Considerations:
1. Baseline Correction: Instruments should have functions to correct for the baseline (background absorbance) to ensure accurate measurements.
2. Reference and Blank Corrections: Use of reference samples and blanks helps in eliminating interference and enhancing accuracy.
3. Maintenance: Regular calibration, cleaning, and maintenance are essential for reliable and accurate measurements.
4. UV-visible spectroscopy is a widely used technique in analytical chemistry, biochemistry, materials science, and various other fields due to its versatility and ability to provide valuable information about molecular structure and composition.
Get in touch with the solution by just clicking the following links-
Facebook Group- www.facebook.com/groups/solutionpharamcy
Mobile App - play.google.com/store/apps/details?id=co.bolton.nq…
New Channel (Pharmacy Dictionary) / @pharmacydictionary
E-Mail for official and other work - [email protected]
#solutionpharmacy #Pharmacologyclass #Pharmacognosyvideos #GPAT
7 ماه پیش
در تاریخ 1402/12/02 منتشر شده
است.
2,552
بـار بازدید شده