Homopolymer Tailing
1.4 هزار بار بازدید -
4 سال پیش
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#Mechanism
#Mechanism
#Examples
It is a technique by which sticky ends can be produced on a blunt-ended DNA molecule. In a homopolymer, all the subunits are same. A DNA strand made up entirely of deoxyguanosine is an example of homopolymer, and is referred to as polydeoxyguanosine or poly(dG).
Tailing involves using the enzyme terminal deoxynucleotidyl transferase to add a series of nucleotides on to the 3'-OH termini of a double-stranded DNA molecule. The reaction when carried out in the presence of just one deoxynucleotide, then a homopolymer tail will be produced.
More videos please click the following links
1. Plasmid Vector pBR322
2. cDNA
3. VITAMIN K I NEET I AIIMS I QUICK RECALL
4. VITAMIN A NEET – AIIMS QUICK RECALL
5. cosmids
6. expression vector
7. Transformation of DNA
#Examples
It is a technique by which sticky ends can be produced on a blunt-ended DNA molecule. In a homopolymer, all the subunits are same. A DNA strand made up entirely of deoxyguanosine is an example of homopolymer, and is referred to as polydeoxyguanosine or poly(dG).
Tailing involves using the enzyme terminal deoxynucleotidyl transferase to add a series of nucleotides on to the 3'-OH termini of a double-stranded DNA molecule. The reaction when carried out in the presence of just one deoxynucleotide, then a homopolymer tail will be produced.
More videos please click the following links
1. Plasmid Vector pBR322
2. cDNA
3. VITAMIN K I NEET I AIIMS I QUICK RECALL
4. VITAMIN A NEET – AIIMS QUICK RECALL
5. cosmids
6. expression vector
7. Transformation of DNA
4 سال پیش
در تاریخ 1399/06/09 منتشر شده
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