Interpretation of HPLC reports for Hemoglobinopathies

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live biochemistry
17 هزار بار بازدید - 2 سال پیش - In this video I have
In this video I have explained about Interpretation of HPLC reports for Hemoglobinopathies in India
This video will be useful for all medical laboratory professional to interprete HPLC chromatograph and diagnose Hemoglobinopathies like sickle trait, sickle disease,beta thalassemia, HbE trait and disease, Hb D Punjab ( Heterozygous and Homozygous) , Hb lepore, HbJ  Meerut, Hb Q India, HPFH and Double Heterozygous conditions in India

It took me three years to learn all different hemoglobinopathies which is covered in just 22 minute video !

#hplcreportinterpretation
#hplc
#hemoglobinopathies
#hplcforhemoglobinopathies
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TIMELINE FOR HPLC CHROMATOGRAPH INTERPRETATION VIDEO :

00:00 Introduction to HPLC chromatograph interpretation

00:11 Brief principle of HPLC

00:52 Common Hemoglobinopathies in India

02:01 Alpha gene variants

02:30 Beta gene variants

03:52 Biorad  variant windows

04:03 Strategies to be followed to diagnose Hemoglobinopathies

05:08 Things to look after in chromatograph

06:45 HbA2 distribution
(Based on concentration different Hemoglobinopathies identified)

07:48 Factors /Diseases affecting HbA2

09:11 Chromatograph of beta thalassemia trait

10:08 Chromatograph of sickle cell trait

10:37 Chromatograph of sickle cell disease

11:17 Chromatograph of HbE trait

11:43 Chromatograph of HbE disease

12:05 Chromatograph of HbD Heterozygous (HbD Punjab)

12:59 Chromatograph of HbD Iran

13:52  Chromatograph of  HbQ India

14:30 Chromatograph of Hb Lepore

15:32 Chromatograph of HbJ Meerut

16:21 Chromatograph of HbC trait

16:42  Windows wise  Hemoglobinopathies (worldwide)

17:41 Examples of double Heterozygous
conditions

19:19 HPFH homozygous / Beta thalassemia major

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Video Highlights
What is normal range of HPLC?
What is HPLC?
How thalassemia diagnosed in HPLC?
What does it mean if HbA2 is high?
What if HbA2 is low?
Hemoglobin pattern analysis
Thalessemia and Hemoglobinopathies testing by HPLC
Detection of abnormal hemoglobin variants by HPLC methods
2 سال پیش در تاریخ 1401/02/16 منتشر شده است.
17,064 بـار بازدید شده
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